Effect of Region and Genipin on Bi-directional Hydrostatic Pressure Resistance of Healthy Vertebral Endplate
Date Issued
2008
Date
2008
Author(s)
Chen, Szusien
Abstract
Objectives. To investigate the effect of regional variation, nucleus material and genipin immersion on the bi-directional fluid flow resistance of healthy vertebral endplate.ummary of Background Data. The exchange of nutrients and wastes for metabolic process in the intervertebral disc can occur via fluid convection through the endplate. The fluid flow can be driven by pressure gradient between the vertebra and the intervertebral disc. However, the factors that influence the resistance of fluid flow of endplate have not been well studied yet. ethods. 125 plugs (diameter: 5mm, length: 6mm) were harvested form the healthy porcine thoracic endplates with a trephine drill. The customized pumping system was used to inject 1mL saline at a speed of 0.0075ml/s through two ends of each plug, simulating the fluid outflow and inflow of the intervertebral disc. During saline injection, the fluid flow resistance was measured as steady state pressure by a pressure transducer connected to the customized pumping system. Three experimental groups were performed to investigate the effect of regional variation, nucleus material and genipine on the fluid flow resistance, respectively. The plugs were harvested from the anterior (n=18), posterolateral (n=18), and central (n=18) endplate for Protocol 1, from the central endplate without removal of nucleus material (n=18) for Protocol 2, and from the central endplate with 18 plugs soaked in genipin solution, 18 plugs soaked in saline, and 17 plugs soaked in distilled water for 1 day for Protocol 3.esults. The resistance of fluid outflow was significantly greater than that of fluid inflow through every region of the endplate. The lowest resistance of fluid inflow/outflow was in the central endplate. The existence of nucleus material increased fluid flow resistance bi-directionally. In comparison with the plugs without being soaked in any solutions, the resistance of fluid outflow increased by 98% (p=0.038), and the resistance of fluid inflow increased by 102% (p=0.089) for the plugs soaked in genipin solution.onclusions. The resistance of fluid flow of the endplate is direction-dependent and region-dependent. Genipin can increase the resistance of fluid outflow and inflow of the endplate.
Subjects
intervertebral disc
endplate
genipin
nutrient
metabolic
permeability
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R95548010-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):08a81808651f753f538646a0cd749131