A Study on Chitosan Nerve Conduit Loading aFGF Microsphere
Date Issued
2009
Date
2009
Author(s)
HSU, CHIA CHUN
Abstract
Nerve bridging is suture a biomaterial-made conduit and to overpass the damaged nerve end to end with microsurgery. Peripheral nerve could be bridged between the proximal nerve and the distal stump to restore the function. Nerve conduits could eliminate tension at the healing site and induce the regeneration of axons. Nerve conduits also could permit neurobiological recovery to enhance neural regeneration and stop cells and their secretions from obstructing neural regeneration. In this study, we provide acid fiberblast growth factor to induce the nerve to be regenerated and use PDLLA microsphere for drug delivery system. Chitosan was used to fabricate the nerve conduit. Combining Freeze-Drying method and dipping in the wet soaking method prepare out the conduit containing two kinds structure , and then rolled the material to make nerve conduit with pore and multi-layered. n the experiment, analyze with SDS page method to know the most stable environment for capsule acid fiberblast growth factor. Use bovine serum albumin as model drug to find the release profile of PDLLA microsphere. And test the best dosage in the cell activity experiment of 3T3 fiberblast cell. Prove acid fiberblast growth factor can be released form the PDLLL microsphere in the ELSA test. Observe chitosan nerve conduit structure by the scanning electron microscope. n The experimental result , ethyl acetate is better at the choice of organic phase than methylene chloride, and add ploy-lysine can promote the stable of the acid fiberblast growth factor undergo double emulsion process. Use bovine serum albumin as model drug to find the release profile of PDLLA microsphere. The result indicate PDLLA microsphere can keep more than 14 day releasing. the best activity dosage of cell is 1-10 ng/ml in the cell activity experiment of 3T3 fiberblast cell.for the release sample of six hours and two days, about 0.7 ng/ml acid fiberblast growth factor can be inspected in ELISA test. We can find the PDLLA microsphere can be kept in the porous layer of the chitosan nerve conduit.Combining Freeze-Drying method and dipping in the wet soaking method can prepare out the conduit containing two kinds structure whose non-porous film layer and 100μm pore sponge layer.
Subjects
Acid Fiberblast Growth Factor
PDLLA
Chitosan
Nerve Conduit
Microsphere
Type
thesis
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