Enhanced pullulan production in a biofilm reactor by using response surface methodology
Journal
Journal of Industrial Microbiology and Biotechnology
Journal Volume
37
Journal Issue
6
Pages
587-594
Date Issued
2010
Author(s)
Abstract
Pullulan is a linear homopolysaccharide that is composed of glucose units and often described as £\-1, 6-linked maltotriose. In this study, response surface methodology using Box-Behnken design was employed to study the effects of sucrose and nitrogen concentrations on pullulan production. A total of 15 experimental runs were carried out in a plastic composite support biofilm reactor. Three-dimensional response surface was generated to evaluate the effects of the factors and to obtain the optimum condition of each factor for maximum pullulan production. After 7-day fermentation with optimum condition, the pullulan production reached 60.7 g/l, which was 1.8 times higher than the result from initial medium, and wasthe highest yield reported to date. The quality analysis demonstrated that the purity of produced pullulan was 95.2%, and its viscosity was 2.5 centipoise (cP), which is higher than the commercial pullulan and related to its molecular weight. Fourier transform infrared spectroscopy (FTIR) also suggested that the produced exopolysaccharide was pullulan. ? Society for Industrial Microbiology 2010.
Subjects
Aureobasidium pullulans
Medium optimization
PCS Biofilm Reactor
Pullulan
Response surface methodology
Type
journal article