Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope
Journal
Optics Express
Journal Volume
21
Journal Issue
25
Pages
31604-31614
Start Page
31604
End Page
31614
ISSN
10944087
Date Issued
2013
Author(s)
Chung, Hsiang-Yu
Kuo, Wei-Cheng
Yu, Che-Hang
Chia, Shih-Hsuan
Lin, Cheng-Yung
Chen, Jie-Shin
Tsai, Huai-Jen
Fedotov, Andrey B.
Ivanov, Anatoly A.
Zheltikov, Aleksei M.
Abstract
In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) of the studied microscope varies from 59 μm × 93 μm up to 178 μm × 280 μm, while the corresponding lateral resolution varies from 0.6 μm to 2 μm for two-photon fluorescence (2PF) signals. With a 34/s video frame rate, in vivo dynamic observation of zebrafish heartbeat through 2PF of the excited green fluorescence protein (GFP) is demonstrated.
Publisher
Optical Society of American (OSA)
Type
journal article