A novel fluorescence quantification method for polymerase chain reaction system
Resource
Optics Communications 266: 744-750
Journal
Optics Communications
Journal Volume
266
Journal Issue
2
Pages
744-750
Date Issued
2006
Author(s)
Chien, Jui Hung
Lee, Da Sheng
Cheng, Yi Ting
Yeh, Shou Huei
Chou, Wen Ping
CHIEN, JH
LEE, DS
CHENG, YT et al.
Abstract
Due to the increasing demand of simultaneous DNA detection, known as multiplexing detection, in a single sample, the current real-time polymerase chain reaction (PCR) platforms are equipped with discrete optics and detectors for different fluorescence wavelength. However, to go beyond four DNA labeling dyes, the optical loss will lower the performance on fluorescence detection. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification. The results show that this prototype provides comparable sensitivity and amplification efficiency as Roche Light-Cycler for DNA quantification and similar reproducibility within five intra assay samples with the DNA quantification method proposed in this study. © 2006 Elsevier B.V. All rights reserved.
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
65.pdf
Size
461.92 KB
Format
Adobe PDF
Checksum
(MD5):a9251b6b0cf2c2f9aaff94d2939f424c