Investigate the biological effect of DNA methylation on G-quadruplex of hTERT gene
Date Issued
2014
Date
2014
Author(s)
Kuan, Yen-Min
Abstract
It has been shown that gene expression will be affected by DNA methylation and secondary structures in the promoter region. However, the gene expression profile has not been addressed when the DNA methylation occurs at the G-quadruplex secondary structures. DNA methylation and G-quadruplex structures has been found in the promoter region of hTERT (human telomerase reverse transcriptase) gene. In this study, we investigated whether the methylation of G-quadruplex will occur at the promoter region of hTERT and further modulate its gene expression level. Using QGRS Mapper (Quadruplex forming G-Rich Sequences), a G-quadruplex structure was identified on the CTCF (CCCTC-binding factor) binding site of hTERT gene. NMR (nuclear magnetic resonance) analysis further confirmed this possible G-quadruplex structure. Reporter plasmids were constructed under the control of different hTERT promoter region. Here we further showed that the expression of hTERT was regulated by the G-quadruplex at the CTCF binding site and DNA methylation at the G-quadruplex further modulate the gene expression.
Subjects
DNA甲基化
G-quadruplex
hTERT
CTCF
Type
thesis
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