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  3. Molecular and Cellular Biology / 分子與細胞生物學研究所
  4. Translation initiation site of mRNA is selected through dynamic interaction with the ribosome
 
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Translation initiation site of mRNA is selected through dynamic interaction with the ribosome

Journal
Proceedings of the National Academy of Sciences of the United States of America
Journal Volume
119
Journal Issue
22
Date Issued
2022
Author(s)
Chen Y.-L
JIN-DER WEN  
DOI
10.1073/pnas.2118099119
URI
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130495447&doi=10.1073%2fpnas.2118099119&partnerID=40&md5=58d99571d695c14f3f2dda0fb08ae378
https://scholars.lib.ntu.edu.tw/handle/123456789/625698
Abstract
SignificanceRibosomes translate the genetic codes of messenger RNA (mRNA) to make proteins. Translation must begin at the correct initiation site; otherwise, abnormal proteins will be produced. Here, we show that a short ribosome-specific sequence in the upstream followed by an unstructured downstream sequence is a favorable initiation site. Those mRNAs lacking either of these two characteristics do not associate tightly with the ribosome. Initiator transfer RNA (tRNA) and initiation factors facilitate the binding. However, when the downstream site forms structures, initiation factor 3 triggers the dissociation of the accommodated initiator tRNA and the subsequent disassembly of the ribosome-mRNA complex. Thus, initiation factors help the ribosome distinguish unfavorable structured sequences that may not act as the mRNA translation initiation site.
Subjects
initiation factors; optical tweezers; single-molecule; smFRET; translation initiation
Other Subjects
initiation factor; messenger RNA; methionine transfer RNA; genetics; metabolism; protein synthesis; ribosome; translation initiation; Peptide Chain Initiation, Translational; Peptide Initiation Factors; Protein Biosynthesis; Ribosomes; RNA, Messenger; RNA, Transfer, Met
Type
journal article

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