Antioxidant Activities and Safety Evaluation of Nano/submicron Lycium spp. suspension
Date Issued
2007
Date
2007
Author(s)
Chan, Ya-Ting
DOI
zh-TW
Abstract
This study discussed the preparation, antioxidant activities, and safety of nano/submicron Lycium spp.. Media milling was used to reduce the material to nano/submicron scale. After milling for 90min，the volume mean diameter decreased to 15.3μm and the number mean diameter decreased to 102 nm. The volume mean diameter after centrifugation (10000 ×g, 10min) was about 587.5 nm. Transmission electron microscopic (TEM) photographs also proved the existence of nano/submicron scale particle. The reducing power, ferrous chelating effect (EC50=2.11 mg/mL) and the Trolox equivalent antioxidant capacity (TEAC) assay (TEAC value=0.028mM) of milled product is higher than that of hot, cold water and 95% ethanol extract. The total polyphenol (19.3 mg/g) , total carotenoids (1.27 mg/g) and β-carotene (0.23 mg/g) contents in milled product was higher than that in the extract by hot, cold water and 95% ethanol. The flavonoids (4.01 mg/g) in milled product was higher than that in the extract by hot, cold water. From Ames test, nano/submicron Lycium suspension in this study had no mutagenic effect to the Samonella typhimurium TA 98 and TA100.
Subjects
枸杞
介質研磨
抗氧化
沙門氏逆突變試驗
Lycium
media milling
antioxidant
Ames test
Type
thesis
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