The Antioxidant Activities and the Inhibitory Effect on Angiotensin I Converting Enzyme Exerted by the Extract of Bacillus subtilis BCRC 14715-fermented Black Soybean
Date Issued
2008
Date
2008
Author(s)
Juan, Ming-Yen
Abstract
Natto, a traditional fermented food in Japan, is prepared by fermenting steamed soybean with Bacillus subtilis. It has been reported to exert antioxidant activities, and the inhibitory effect on angiotensin I converting enzyme (ACE) as well. In the present study, various solvent (water, 80% methanol, 80% ethanol, 80% acetone) extracts of B. subtilis BCRC 14715-fermented black soybean were examined for total phenolic, flavonoid, and anthocyanin contents as well as the antioxidant activities including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals scavenging effects, reducing power, and Fe2+-chelating ability. Besides, effect of fermentation period and extraction solvent on ACE inhibitory activity of the Bacillus spp.-fermented black soybean extracts were also investigated.he results showed that the extract of the fermented black soybean exhibited significantly higher (p<0.05) total phenolic, flavonoid, anthocyanin contents and antioxidant activities than did the extract of the nonfermented black soybean. Among the various extracts examined, acetone extract showed the highest contents of total phenolic, flavonoid and anthocyanin. The methanol extract excerted the highest Fe2+-chelating ability, while the acetone extract showed the highest DPPH free radicals scavenging effects and reducing power.he inhibitory activity on ACE of bean and viscous material extracts of Bacillus spp.-fermented black soybean increased as the fermentation period was increased. Bacillus sp. CN11-fermented black soybean extract exhibited the highest ACE inhibitory activity. A higher ACE inhibitory activity was noted in bean extracted with 80% ethanol than with water. On the other hand, a significantly higher (p<0.05) ACE inhibitory activity was noted in the water extract of viscous material than the 80% ethanol extract.
Subjects
black soybean
extraction solvent
antioxidant activity
angiotensin I converting enzyme (ACE)
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R95641027-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):b1be961773f5239a836e253618b426c2