Cloning and characterization of a cDNA encoding an 18.0-kDa class-I low-molecular-weight heat-shock protein from rice
Journal
Gene
Journal Volume
165
Journal Issue
2
Pages
223-227
Date Issued
1995
Date
1995
Author(s)
Lee, Yueh-Luen
Chang, Pi-Fang L.
Yeh, Kai-Wun
Kung, Cheng-Che S.
Lin, Wan-Chi
Chen, Yih-Ming
Lin, Chu-Yung
Abstract
A novel cDNA clone, Oshp18.0 cDNA, encoding a rice (Oryza sativa L. cv. Tainong 67) 18.0-kDa heat-shock protein (HSP), was isolated from a cDNA library of heat-shocked rice seedlings by use of the rice HSP cDNA, Oshsp17.3 cDNA, as a probe. The sequence showed that Oshsp18.0 cDNA contains a 749-bp insert encoding an ORF of 160 amino acids, with a predicted molecular mass of 18.0 kDa and a pI of 7.3. Sequence comparison reveals that Oshsp18.0 cDNA is highly homologous to other low-molecular-weight (LMW) HSP cDNAs. Also, the results of hybrid-selected in vitro translation clearly establish that Oshsp18.0 cDNA is the rice 18.0-kDa LMW HSP-encoding cDNA clone. The recombinant Oshsp18.0 fusion protein produced in Escherichia coli was of the size predicted, and was recognized by the class-I rice 16.9-kDa HSP antiserum. The results suggest that Oshsp18.0 cDNA is an 18.0-kDa class-I LMW HSP- encoding cDNA clone from rice.
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
04.pdf
Size
729.24 KB
Format
Adobe PDF
Checksum
(MD5):f81ef1a10558a4550a7bb8cf18ae3277