To Promote Chondrogenic Differentiation of Human Mesenchymal Stem Cells on Chitosan/PCL blended substrates
Date Issued
2009
Date
2009
Author(s)
Ho, Chia-Chi
Abstract
In different environment, the cellular morphology and shape will affect its physiological function, such as differentiation and proliferation. Therefore, it is a niche to control the physiological function of cell using cultural substrate to regulate the cellular attachment which could induce morphology and shape. Two biodegraded materials (e.g. chitosan and PCL), which were approved by Food and Drug Administration (FDA, US) were used in this study. Chitosan is wildly employed as artificial skin and cartilage tissue engineering. However, some studies indicated that chitosan was not a suitable material for fibroblast attachment and proliferation. Another material, PCL is also wildly used in suture and lots of studies demonstrated that the PCL could promote fibroblast attachment and growth. We would like establish a model of ability of cell adhesion, proliferation rate, and possibility of MSCs differentiate into chondrocyte when cultured on PCL and chitosan blended substrates. In this study, we try to mix chitosan and PCL which could then modulate the adhesive on ability of cells. Further, to promote the function of chondrogenic differentiation of stem cells by altering cell morphology and cell shape.
Subjects
chitosan
PCL
blended materials
mesenchymal stem cells
chondrogenic differentiation
Type
thesis
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