Multifunctional nanocarrier as a potential micro-RNA delivery vehicle for neuroblastoma treatment
Journal
Journal of the Taiwan Institute of Chemical Engineers
Journal Volume
96
Pages
526-537
Date Issued
2019
Author(s)
Abstract
Neuroblastoma accounts for about 6% of all cancers in children, with a 5¡Vyear survival rate of only 20¡V25%. The increased expressin of MYCN is associated with poor prognosis in patients with neuroblastoma. MicroRNA¡V34 (miR¡V34) may serve as a potential target for cancer treatment, owing to its function as an oncogene and tumor suppressor. In this study, positively charged magnetic nanocarriers comprising cross-linked polyethylenimine (PEI)¡Vtripolyphosphate (TPP)¡Vcoated iron oxide nanoparticles (IONPs) were developed using co-precipitation method. These nanocarriers were capable of penetrating the cell wall and used for the delivery of miRNA¡V34a into the cells. The diffraction peak of synthesized magnetic nanocarriers at 2£c = 35.44¢X corresponded to that of magnetite (Fe 3 O 4 ) (311), consistent with Joint Committee on Powder Diffraction Standards (JCPDS) database. The characteristic peaks at 3380, 1620, 2900, and 2840/cm corresponded to those of NH and ? CH 2 ? groups, indicating the successful coating of PEI. In addition, the small angle neutron scattering (SANS) spectra showed that the alternative magnetic field (AMF) triggered core heat generation, which softened the shells. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed the nontoxicity of these nanocarriers to BE¡V2¡VM17 cells. The expression of miR-34a was raised and the expression of MYCN, the target of miR-34a, was significantly suppressed. ? 2018 Taiwan Institute of Chemical Engineers
Subjects
Iron oxide nanoparticle
MicroRNA-34
MYCN
Neuroblastoma
Polyethylenimine
Tripolyphosphate
SDGs
Other Subjects
Diagnosis; Diffraction; Diseases; Magnetite; Metal nanoparticles; Nanomagnetics; Neutron scattering; Precipitation (chemical); RNA; Iron oxide nanoparticle; MicroRNAs; MYCN; Neuroblastomas; Polyethylenimines; Tripolyphosphates; Iron oxides
Type
journal article