Surface-modified cellulose paper and its application in infectious disease diagnosis
Journal
Sensors and Actuators B: Chemical
Journal Volume
265
Pages
506--513
Date Issued
2018-07
Author(s)
Abstract
A sensitive and stable cellulose paper platform modified with carboxymethyl cellulose (CMC) was investigated for use as a rapid and accurate immunoassay for tuberculosis. Absorbing CMC to the paper substrate enables it to act as a linker for immobilizing biomolecules in a hydrophilic environment to promote their functionality and stability. This CMC-modified cellulose material can also be crosslinked with 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride/N-Hydroxy-succinimide (EDC/NHS) to generate NHS ester groups on the paper surface that can be covalently conjugated with ligands or target analytes for highly specific binding. As a result, with this system we were able to achieve a detection limit of 0.03 ng/mL for tuberculin-purified protein derivative. In addition, multiple tests can be completed simultaneously in as little as 21 min. These results demonstrate how this surface-modified paper analytical device can provide sensitive and high-throughput on-site disease diagnosis for resource-limited settings. ? 2018 Elsevier B.V.
Subjects
Colorimetric assay; Paper-based analytical device; Surface chemistry; Tuberculosis diagnosis
SDGs
Other Subjects
Analytic equipment; Cellulose; Chemical analysis; Crosslinking; Surface chemistry; Carbodiimide hydrochlorides; Carboxymethyl cellulose; Colorimetric assays; Hydrophilic environments; Infectious disease; Modified cellulose; Paper-based analytical devices; Tuberculosis diagnosis; Diagnosis; Carboxymethyl Cellulose; Cellulose; Chemical Analysis; Diagnosis; Ester Groups; Paper
Type
journal article