Dynamic visualization of the recovery of mouse hepatobiliary metabolism to acetaminophen-overdose damage
Journal
Journal of Biophotonics
Journal Volume
12
Journal Issue
3
Date Issued
2019
Author(s)
Li, Feng-Chieh
Lin, Hung-Ming
Lin, Hung-Ming
Lin, Chih-Ju
Lin, Chih-Ju
Wang, Sheng-Shun
Wang, Sheng-Shun
Lee, Yu-Yang
Lee, Yu-Yang
Lo, Su-Yen
Lo, Su-Yen
Sun, Tzu-Lin
Sun, Tzu-Lin
Chen, Wei-Liang
Chen, Wei-Liang
Lo, Wen
Lo, Wen
Horton, Nicholas
Horton, Nicholas
Xu, Chris
Xu, Chris
Chiang, Shu-Jen
Chiang, Shu-Jen
Chen, Yang-Fang
Chen, Yang-Fang
Lee, Hsuan-Shu
Lee, Hsuan-Shu
Abstract
Acetaminophen (APAP) overdose is one of the world's leading causes of drug-induced hepatotoxicity. Although traditional methods such as histological imaging and biochemical assays have been successfully applied to evaluate the extent of APAP-induced liver damage, detailed effect of how APAP overdose affect the recovery of hepatobiliary metabolism and is not completely understood. In this work, we used intravital multiphoton microscopy to image and quantify hepatobiliary metabolism of the probe 6-carboxyfluorescein diacetate in APAP-overdose mice. We analyzed hepatobiliary metabolism for up to 7 days following the overdose and found that the excretion of the probe molecule was the most rapid on Day 1 following APAP overdose and slowed down on Days 2 and 3. On Day 7, probe excretion capability has exceeded that of the normal mice, suggesting that newly regenerated hepatocytes have higher metabolic capabilities. Our approach may be further developed applied to studying drug-induced hepatotoxicity in vivo.
SDGs
Publisher
Wiley-VCH Verlag
Type
journal article