Comparison of abbott realtime HCV genotype II with versant line probe assay 2.0 for hepatitis C virus genotyping
Journal
Journal of Clinical Microbiology
Journal Volume
53
Journal Issue
5
Pages
1754-1757
Date Issued
2015
Author(s)
Liang C.-C
Lin C.-L
Abstract
Genotyping and subtyping of 225 samples with hepatitis C virus (HCV) genotype 1, 2, 3, or 6 infection were done with Versant LiPA 2.0 and Abbott RealTime HCV Genotype (GT) II by using direct sequencing of the NS5B and 5= untranslated regions as the reference standards. The concordance rates were>99.2% for genotypes and 96.1% for subtypes 1a and 1b. Both the Abbott Real- Time and Versant LiPA assays can accurately determine hepatitis C virus genotypes. (This study has been registered at Clinical- Trials.gov under registration no. NCT00979979.) Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
SDGs
Other Subjects
nonstructural protein 5B; 5' untranslated region; adult; aged; analytical equipment; Article; assay; controlled study; diagnostic accuracy; female; genotyping technique; hepatitis B; Hepatitis C virus; Hepatitis C virus genotype 1; Hepatitis C virus genotype 2; Hepatitis C virus genotype 3; Hepatitis C virus genotype 6; Hepatitis C virus subtype 1a; Hepatitis C virus subtype 1b; human; hybridization; intermethod comparison; limit of detection; line probe assay; major clinical study; male; mixed infection; nonhuman; NS5B gene; polymerase chain reaction; priority journal; real time polymerase chain reaction; retrospective study; reverse hybridization; classification; comparative study; evaluation study; genetics; genotyping technique; Hepacivirus; hepatitis C; isolation and purification; middle aged; molecular diagnosis; procedures; virology; young adult; Hepatitis C virus; Hepatitis C virus genotype 1; Adult; Aged; Female; Genotyping Techniques; Hepacivirus; Hepatitis C; Humans; Male; Middle Aged; Molecular Diagnostic Techniques; Retrospective Studies; Young Adult
Publisher
American Society for Microbiology
Type
journal article