Development of InDel Markers for Variety Identification amomg Major Japonica Rice Cultivars in Taiwan
Date Issued
2007
Date
2007
Author(s)
Yen, Tsai Yi
DOI
zh-TW
Abstract
In order to identify the major japonica rice cultivars in Taiwan, 10 single nucleotide polymorphisms (SNP) markers, 152 insertion/deletion (InDel) markers and 156 sequence tagged sites (STS) markers were screened against 21 japonica rice cultivars. Among the 318 markers screened, 59 of them are polymorphic, including 4 SNP markers, 30 InDel markers, and 25 STS markers. After eliminating dominant markers, tetra primer markers and replications of polymorphisms, there are 34 co-dominant markers showing unique polymorphic patterns. Five minimum sets of markers that can identify each of the 21 japonica rice cultivars completely from these 34 unique polymorphic markers contains only 7 markers. The sensitivity for testing bulk samples reach 10% while using these co-dominant markers, and therefore suitable for analyzing large amount of samples during variety testing.
Subjects
稉
稻
分子標記
品種鑑別
japonica rice
marker
variety Identification
Type
thesis
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