Analysis of Staphylococcal Enterotoxins from Workers- and Livestock Animals-Isolates
Date Issued
2005
Date
2005
Author(s)
Chen, Ren-Shinn
DOI
zh-TW
Abstract
This study analyzed 563 suspected Staphylococci isolates from poultry farms, swine herds, bovine herds previously, and newly sampling isolates from meat market in order firstly to distinguish Staphylococcus aureus and then to characterize oxacillin resistant Staphylococcus aureus (ORSA), types of enterotoxin production and bacterial genetic similarity. Staphylococci were mainly collected from chicken, cattle and human origins. Twenty-three ORSAs were identified by use of screening plate test and 20 of these strains were from human source. A higher contamination potential from human origin was thus suspected. Five types of bacterial enterotoxin (A to E) were detected by using Staphylocooccal Enterotoxin Rreversed Passive Latex Agglutination (SET-RPLA) and also by five groups of polymerase chain reaction (PCR) primers. Twenty-one enterotoxin producing strains were identified. The type of enterotoxins were type B 38% (8/21), type A 24% (5/21), type C 24% (5/21) and type A&B 14% (3/21). Fifteen ORSA strains composed 3 strains of type A&B, 2 strains of type A, 6 strains of type B and 4 strains of type C, and 6 non-ORSA Staphylococci producing toxins composed 3 strains of type A, 2 strains of type B and 1 type C. No type D and E toxin producing strain were detected. The agreement of detection between RPLA and PCR were only 69% and PCR had a better sensitivity. Genetic similarity of staphylocooccal enterotoxin strains and ORSA strains by using pulse-field electrophoresis indicated that human source strains collected from poultry farm were high. Three enterotoxin strains from the same poultry farm had highest similarity that was due to close-raising environment. Genetic similarities of bacteria collected from meat market were low, and that might due to open environment and high diversity of animal and human from different origins. Workers from meat market with shorter working experience had higher isolation percentage of ORSA and staphylocooccal enterotoxin strains but with no statistical differences (P > 0.05). The isolation was also found to be no difference between workers’ working environment in meat market. The study suggests a necessary continuous monitoring of ORSA and staphylocooccal enterotoxin strains in worker at farm and meat market, and also need to analyze the relationship between environment, human and animals.
Subjects
葡萄球菌腸毒素
聚合酶
鏈鎖反應
脈衝式膠體電泳
staphylococcal enterotoxin
PCR
PFGE
Type
thesis
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