Preparation of Porous Scaffolds by Using Freeze-Extraction and Freeze-Gelation Methods
Resource
Biomaterials 25: 129-138
Journal
Biomaterials
Pages
129-138
Date Issued
2004
Date
2004
Author(s)
Ho, Ming-Hua
Liu, Hwa-Chang
Hsien, Tzu-Yang
Lai, Juin-Yih
Hou, Lein-Tuan
Abstract
Freeze-fixation and freeze-gelation methods are presented in this paper which can be used to prepare highly porous scaffolds without using the time and energy consuming freeze-drying process. The porous structure was generated during the freeze of a polymer solution, following which either the solvent was extracted by a non-solvent or the polymer was gelled under the freezing condition; thus, the porous structure would not be destructed during the subsequent drying stage. Compared with the freeze-drying method, the presented methods are time and energy-saving, with less residual solvent, and easier to be scaled up. Besides, the problem of formation of surface skin can be resolved and the limitation of using solvent with low boiling point can be lifted by the presented methods. With the freeze-extraction and freeze-gelation methods, porous PLLA, PLGA, chitosan and alginate scaffolds were successfully fabricated. In addition to the presentation of the morphologies of the fabricated scaffolds, preliminary data of cell culture on them are as well included in the present work. © 2003 Elsevier Ltd. All rights reserved.
Subjects
Freeze-drying; Freeze-extraction; Freeze-gelation; Scaffold; Tissue engineering
SDGs
Other Subjects
Cell culture; Drying; Energy utilization; Freezing; Gelation; Morphology; Scaffolds; Energy saving; Biomaterials; alginic acid; chitosan; polyglactin; polylactic acid; polymer; animal cell; article; biocompatibility; biodegradability; controlled study; energy conservation; fibroblast culture; freeze drying; freezing; gelation; intermethod comparison; nonhuman; polymerization; porosity; priority journal; rat; scale up; skin fibroblast; skin surface; solvent effect; solvent extraction; temperature dependence; time; tissue engineering
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
05.pdf
Size
955.76 KB
Format
Adobe PDF
Checksum
(MD5):5165eb6413f32e06d240006664846f49