Chapter 16 Advanced Gene Mapping in Eukaryotes
Date Issued
2000
Date
2000
Author(s)
Wang, Yue-Wen
DOI
246246/2006092815540093
Abstract
1. In some haploid eukaryotic organisms (fungi or single-celled algae) products of a single meiosis, the meiotic tetrad, are contained within one structure. Tetrad analysis provides insight into meiotic events.
2. In haploid organisms, the phenotype correlates directly with the genotype of each member of the tetrad (no dominance or recessiveness occurs).
3. Life cycles of organisms typically used in tetrad analysis:
a. Saccharomyces cerevisiae (baker’s yeast), has two mating types, MATa and MATα (Figure 16.1).
i. Asexual reproduction occurs mitotically (vegetative life cycle) in the haploid yeast.
ii. Sexual reproduction, fusion of a haploid a cell with a haploid α one, produces a diploid cell (a/α) that also reproduces mitotically, giving rise to identical diploid cells.
iii. Diploid cells sporulate by meiosis, producing four haploid ascospores contained in an ascus. Of the ascospores, two will be type a and two type a. In yeast they are unordered tetrads, arranged randomly in the ascus (Figure 13.16).
Publisher
臺北市:國立臺灣大學農藝學系
Type
learning object
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