Identification of Meat Species by Amplifying Partial Cytochrome b Gene Fragments for Domestic Dogs, Domestic Cats and Nutria
Date Issued
2006
Date
2006
Author(s)
Chung, Chia-Ju
DOI
en-US
Abstract
Many dogs and cats are slaughtered in East-Asia because of the needs in their meat for food or other purposes. Identification of the species is required to determine that a crime against Animal Protection Law in Taiwan has been committed. Otherwise, some harmful species like nutria (Myocastor coypus) has invaded Taiwan far from the place they originally inhabit, and may damage the ecological system of wetland in Taiwan gradually. This thesis details a good DNA technique using part of the cytochrome b gene on the mitochondrial genome that can work on poor-quality samples such as decayed or over-cooked meat. An appropriate deoxyribonucleic acid (DNA) extraction technique is required to obtain at least 15 ng of DNA. Amplification of part of the cytochrome b gene using specific primers produces a fragment of 252/292/297 bp in size. Direct sequencing of the PCR products allows confirmation of the DNA sequences to those already described on the GENbank-EMBL DNA database to ensure the results of identification.
Subjects
狗肉
貓肉
美洲巨水鼠
肉品
種別鑑定
dog meat
cat meat
nutria
identification
Type
thesis
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