Association between endothelial nitric oxide synthase polymorphisms and the risk of premature coronary artery disease in Taiwan
Journal
Journal of Internal Medicine of Taiwan
Journal Volume
14
Journal Issue
1
Pages
1-10
Date Issued
2003
Author(s)
Abstract
Endothelial dysfunction is one of the initiating processes in atherosclerosis. Endothelium-derived nitric oxide, generated by endothelial nitric oxide synthase (eNOS), is important in endothelial function. Two polymorphisms in eNOS, 894 G→T in exon 7 and a 27-bp repeat in intron 4, have been proposed to be important in coronary artery disease (CAD). We hypothesized that eNOS polymorphisms might be associated with increased risk for premature CAD in Taiwan and susceptibility to endothelial dysfunction. We enrolled 114 patients, including 108 males who were less than 45 years old with significant coronary stenosis, or below 55 years old with documented myocardial infarction (MI), and 6 premenopausal women with CAD or MI. Sixty-five age-matched subjects with patent coronary angiograms were included as controls. The eNOS polymorphisms were examined. Thirty subjects with patent coronary arteries underwent evaluation of brachial artery reactivity in response to hyperemia and sublingual nitroglycerin (NTG). The distributions of 894 G→T in exon 7 and the 27-bp repeat in intron 4 genotypes did not differ significantly between the premature CAD and control groups. Male sex and hyperlipidemia were independent risk factors for premature CAD in Taiwan. Neither reactive hyperemia nor NTG-mediated vasodilatation showed any difference between genetic polymorphs. In terms of impaired flow-mediated vasodilatation, hypertension was the independent factor (p=0.044), and hyperlipidemia had a marginal effect (p=0.056) when we used a multivariate linear regression model. Neither the rare eNOS T nor the 4a allele conferred an independent risk factor for premature CAD in Taiwan. In addition, they had little influence on the endothelial function assessed by flow-mediated vasodilatation.
SDGs
Other Subjects
glyceryl trinitrate; nitric oxide; nitric oxide synthase; adult; allele; angiocardiography; atherosclerosis; blood vessel reactivity; brachial artery; controlled study; coronary artery blood flow; coronary artery disease; coronary artery obstruction; coronary risk; disease association; endothelium; enzyme polymorphism; exon; female; gender; genotype; heart infarction; human; hyperemia; hyperlipidemia; hypertension; hypothesis; intron; linear regression analysis; major clinical study; male; nucleotide sequence; premenopause; review; risk factor; statistical model; Taiwan; vasodilatation
Type
review