以植物細胞培養技術生產重組塵過敏原蛋白Der p2之研究
Date Issued
2005-07-31
Date
2005-07-31
Author(s)
DOI
932313B002086
Abstract
Recombinant allergenic proteins have been produced in a variety of different expression systems. Plants and plant cells
are now considered as viable and competitive expression systems for large-scale protein production. In this research, the
plantlet of transgenic tobacco contained CaMV 35S promoter chimeric with Dermatophagides pteronyssinus 2 (Der p 2)
gene was used to induce formation of callus and hairy roots, and were studied for recombinant Der p 2 production. By Der
p 2 sandwich enzyme-linked immunosorbent assay (sandwich-ELISA), cell line S3 expressed the highest Der p 2
productivity in 8 suspension cell lines. The maximum productivity in flasks was 788.3 µg/L; batch culture in bioreactor
reached the highest productivity, 515.1 µg/L. Among 24 cell lines of hairy roots from transgenic tobacco, maximum
productivity was 475.2 ng/mL. After the treatment of ammonia sulfate precipitation, cation exchange chromatography, and
monoclonal anti-Der p 2 affinity column, recombinant Der p 2 protein was purified into homologous, the recovery rate was
19.7%.
Publisher
臺北市：國立臺灣大學生化科技學系
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
932313B002086.pdf
Size
368.77 KB
Format
Adobe PDF
Checksum
(MD5):8d2320bcc92d5c95ae8972dc15ae94d7