周邊血液單核細胞內C型肝炎病毒之分子病毒學研究(2/2)
Date Issued
2000
Date
2000
Author(s)
高嘉宏
DOI
892315B002009
Abstract
With the recent advent of cloning a novel hepatitis C virus (HCV) and subsequent development
of several serological assays, at present HCV has been recognized as the major etiologic agent of
parenteral non-A, non-B hepatitis worldwide [6, 14, 44, 45, 54]. In order to clarify the biological
significance and clinical implications of HCV in PBMC from patients with chronic type C
hepatitis, we have conducted a series of studies including (1) quantitation of HCV in serum as
well as in PBMC and estimation of the frequency of HCV-infected PBMC; (2) whether diversity
of virus quasispecies exists between serum and PBMC; (3) whether specific sequences for
receptor recognition exist in the HVR-1 region of HCV genome to determine the PBMC tropism
in the past two years. Our results showed that (1) the average serum HCV titer is 1.5x10 4
copies/mL and the average titer of positive-strand and negative-strand HCV RNA in PBMC is
7.8x10 2 and 2.7x10 2 copies/ug RNA. In addition, the titer of positive-strand HCV RNA in PBMC
is 1 to 10 times higher than that of negative-strand HCV RNA in PBMC, suggesting a low
replication efficiency of HCV in such cells. (2) The quasispecies complexity of HCV genome in
serum and PBMC is 5.4+1.7 and 7.0+2.5, suggesting different selection pressure in different
tissue compartments. (3) The aa 402(L) of HVR-1 may be associated with the PBMC tropism of
HCV; however, further studies are warranted to confirm this preliminary finding. Based on these
results, we could understand, at least in part, the pathogenesis of viral persistence and cell
tropism of HCV. In the meantime, these data will shed much light on the establishment of culture
system for HCV infection and will significantly influence our future strategies of hepatitis C
vaccine development and antiviral therapy.
Subjects
Hepatitis C virus
peripheral blood mononuclear cell
HCV
PBMC
SDGs
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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