Detection of Herpesvirus anguillae infection in eel using in situ hybridization
Resource
Journal of Applied Ichthyology 19 (2): 99-103
Journal
Journal of Applied Ichthyology
Journal Volume
2
Journal Issue
19
Pages
99-103
Date Issued
2003
Date
2003
Author(s)
Abstract
A nucleic acid probe for the Herpesvirus anguillae (HVA) Taiwan isolate was constructed using recombinant DNA techniques. This probe consisted of a specific viral DNA fragment (1550 bp) generated by digestion of HVA DNA with the restriction enzyme HindIII, and labeled non-radioactively with digoxigenin (DIG). The probe was used to detect the HVA genome from HVA-infected cell cultures and tissue specimens prepared from infected cels, using either dot blot or in situ hybridizations.
Other Subjects
detection method; eel; genetic analysis; viral disease; Anguilla anguilla; Anguilliformes; Herpesviridae
Type
journal article
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