The molecular genetics of the mouse Iβ-1,6-N-acetylglucosaminyltransferase locus
Resource
Biochemical and Biophysical Research Communications 303 (3): 868-876
Journal
Biochemical and Biophysical Research Communications
Journal Volume
303
Journal Issue
3
Pages
868-876
Date Issued
2003
Date
2003
Author(s)
Abstract
The I antigen and its precursor, the i antigen, are carbohydrate structures and are found on the surface of most mammalian cells. Conversion of the i to the I structure requires I β-1,6-N-acetylglucosaminyltransferase activity. The present investigation demonstrates a novel transcript form expressed from the mouse I locus and elucidates the molecular genetics and the genomic organization of the mouse I locus. The mouse I locus was demonstrated to express three transcript forms, one newly identified and two previously reported, which have a different exon 1 but identical exons 2 and 3. The three transcripts were shown to express differentially in various mouse tissues, and all their protein products demonstrated GlcNAc-transferring activity in enzyme function assay. The molecular genetics proposed for the mouse I locus shows that it is homologous to the human I locus. It has been established recently that a defect in the human I locus may lead to the development of congenital cataracts. It was demonstrated that the mouse and the human I transcripts expressed in the epithelium cells of the mouse and human lens, respectively, are homologous forms. © 2003 Published by Elsevier Science (USA).
Subjects
I locus; IGnT; Lens; β-1,6-N-acetylglucosaminyltransferase
Other Subjects
beta 1,6 n acetylglucosaminyltransferase; blood group I antigen; n acetylgalactosamine; protein; transferase; unclassified drug; animal cell; article; congenital cataract; controlled study; enzyme activity; epithelium cell; gene expression; gene locus; genetic organization; genetic transcription; genome; human; male; molecular genetics; mouse; mouse strain; nonhuman; nucleotide sequence; priority journal; protein function; sequence homology; Mammalia
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
04.pdf
Size
802.47 KB
Format
Adobe PDF
Checksum
(MD5):5d0ac52e2ae34605ab494155ed64ed46