The Diagnosis of Silkworm Disease
Date Issued
2007
Date
2007
Author(s)
Lo, Chu-Min
DOI
zh-TW
Abstract
The viral studies need to establish in vitro viral propagation, and the morphological and molecular data are necessary for the identification and classification of an unknown virus. The aim of this study was undertaken to identify the viral pathogen from moribund silkworm larvae. For in vitro propagation, we selected a high NPV susceptible cell strain, LY16, from NTU-LY1 cells. The tissue fluid extracted from the moribund silkworm larvae was used as an inoculum to infect LY16 cells. The infected LY16 cells were examined by light and electron microscopes. The viral inclusion bodies and virogenic stroma were found in the cytoplasm of the cells. The gross anatomies of the disease silkworm larvae fed or injected with the media of the infected LY16 cells showed a typical symptom of flacheries disease. The columnar cells of the midgut of the disease silkworm were main target cells of the virus and also the muscle cells surrounded the midgut were seriously infected. The purified viral particles are icosahedral in shape and 28 nm in size by negative stain. Based on cytopathic effect and electropathogenic observations, this virus is highly similar to PnV (Perina nuda virus). By RT-PCR amplification, we found that PnV exists in uninfected LY16 cells, therefore LY16 cells are persistent infection with PnV. In conclusions, (1) LY16 cells are PnV-persistent infection cells; (2) PnV can infect both LY cells and silkworm larvae; (3) and the origin pathogen of silkworm may either PnV contaminated from lab during rearing or viral pathogens of silkworm origin which can’t propagate in LY16 cells.
Subjects
榕樹透翅毒蛾病毒
軟化症病毒
家蠶病毒病
細胞株
病原體
Perina nuda virus (PnV)
infectious flacherie virus ( IFV)
the viral silkworm disease
cell line
pathogen
Type
thesis
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