A novel DNA chromatography method to discriminate Mycobacterium abscessus subspecies and macrolide susceptibility
Journal
EBioMedicine
Journal Volume
64
Date Issued
2021-02
Author(s)
Yoshida, Mitsunori
Sano, Sotaro
Fukano, Hanako
Suzuki, Masato
Asakura, Takanori
Morimoto, Kozo
Murase, Yoshiro
Miyamoto, Shigehiko
Kurashima, Atsuyuki
Hasegawa, Naoki
Mitarai, Satoshi
Ato, Manabu
Hoshino, Yoshihiko
Abstract
The clinical impact of infection with Mycobacterium (M.) abscessus complex (MABC), a group of emerging non-tuberculosis mycobacteria (NTM), is increasing. M. abscessus subsp. abscessus/bolletii frequently shows natural resistance to macrolide antibiotics, whereas M. abscessus subsp. massiliense is generally susceptible. Therefore, rapid and accurate discrimination of macrolide-susceptible MABC subgroups is required for effective clinical decisions about macrolide treatments for MABC infection. We aimed to develop a simple and rapid diagnostic that can identify MABC isolates showing macrolide susceptibility.
Subjects
ATS/ERS/ESCMID/IDSA guideline; DNA chromatography; Drug susceptibility; Non-tuberculosis mycobacteria; Subspecies discrimination; Whole genome sequencing
Other Subjects
azithromycin; clarithromycin; erythromycin; macrolide; RNA 16S; antiinfective agent; DNA; macrolide; antibiotic resistance; Article; bacterial strain; bacterium isolate; chromatography; DNA extraction; DNA sequence; drug sensitivity; genetic marker; genotype; limit of detection; multiplex polymerase chain reaction; Mycobacterium abscessus; nonhuman; phylogeny; polymerase chain reaction; sensitivity and specificity; sequence analysis; Taiwan; whole genome sequencing; bacterial gene; chromatography; classification; drug effect; genetics; human; microbial sensitivity test; Mycobacterium abscessus; procedures; Anti-Bacterial Agents; Chromatography; DNA; Drug Resistance, Bacterial; Genes, Bacterial; Humans; Macrolides; Microbial Sensitivity Tests; Mycobacterium abscessus; Phylogeny; Polymerase Chain Reaction
Type
journal article