Activation of topoisomerase II-mediated excision of chromosomal DNA loops during oxidative stress
Journal
Genes and Development
Journal Volume
13
Journal Issue
12
Pages
1553-1560
Date Issued
1999
Author(s)
Abstract
Hydrogen peroxide (H2O2), a reactive oxygen species (ROS), is known to induce oxidative stress and apoptosis. U937 cells treated with H2O2 were shown to produce high molecular weight (HMW) DNA fragments approximately 50 to 100 kb in size in <1 min. The formation of these HMW DNA fragments is reversible and shown to be mediated by DNA topoisomerase II (TOP2). Following this initial event, formation of irreversible HMW DNA fragments and nucleosomal ladders occurs. Our results thus demonstrate a potential role of TOP2 in oxidative damage of DNA and apoptotic cell death.
Publisher
Cold Spring Harbor Laboratory Press
Type
journal article