行政院國家科學委員會專題研究計畫期中進度報告:有機污染物綠色螢光蛋白生物偵測器之研發及應用(1/2)
Date Issued
2004
Date
2004
Author(s)
DOI
922211E002054
Abstract
A biosensor is composed of a biological sensing component, which recognizes chemical or
physical changes, couple to a transducing element that produces a measurable signal in response to
the environmental insult. Cell-based biosensors can be classified according to the response of
their sensing element, such as changes in cellular metabolism, pH, altered gene expression in
genetically modified organisms. Genetically engineered cell-based biosensors can elicit a
response in the presence of an environmental insult by coupling the sensing element to a reporter
gene fusion, which upon expression produces a readily measurable signal. The sensing element is
often composed of regulatory proteins and promoter. The specificity of these sensing elements for
the environmental insult confers selectivity to the system, while the reporter protein determines the
system’s sensitivity and detection limits.
We proposed the construction, laboratory characterization, and environmental testing of
genetically engineered GFP-based bacterial biosensors that detect specific organic pollutants (e.g.,
benzene, naphthalene, salicylate, PCBs, and chlorocatechols). The reporter plasmid in which
expression of gfp gene for jellyfish Aequorea victoria green fluorescence protein (GFP) will be
placed directly under the control of the specific promoter. The primary advantage of GFP as a
reporter protein is its autofluorescence, and therefore, its use does not require the addition of
cofactors or exogenous substracts to produce fluorescence. Additionally advantages of GFP
included high stability at biological pH, assay simplicity, and lack of endogenous homologues in
most target organisms. Therefore, such GFP-based biosensors might provide even real time
monitoring environmental pollutants.
This is a two-year project. In the first year, the main task is to establish a sensitive and stable
biosensing system using GFP as reporter protein. In addition, the benzene and related compound
biosensor will be developed. The dose-dependent and time-dependent relationship of the
biosensor and organic compound will be investigated. Furthermore, the application of biosensor
in detecting environmental will be explored. Currently, we have successfully developed the
recombinant biosensor plasmids. Other investigations are in progress.
Subjects
biosensors
green fluorescence protein (GFP)
organic pollutants
SDGs
Publisher
臺北市：國立臺灣大學生物環境系統工程學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
922211E002054.pdf
Size
127.11 KB
Format
Adobe PDF
Checksum
(MD5):a3109c8b4733038b1cccd85a5051273b