Mass spectrometric detection of nanoparticle host-guest interactions in cells
Journal
Analytical Chemistry
Journal Volume
86
Journal Issue
13
Pages
6710-6714
Date Issued
2014
Author(s)
Yan B.
Yesilbag Tonga G.
Hou S.
Fedick P.W.
Yeh Y.-C.
Alfonso F.S.
Mizuhara T.
Vachet R.W.
Rotello V.M.
Abstract
Synthetic host-guest chemistry is a versatile tool for biomedical applications. Characterization and detection of host-guest complexes in biological systems, however, is challenging due to the complexity of the biological milieu. Here, we describe and apply a mass spectrometric method to monitor the association and dissociation of nanoparticle (NP)-based host-guest interactions that integrates NP-assisted laser desorption/ionization (LDI) and matrix assisted laser desoption/ionization (MALDI) mass spectrometry. This LDI/MALDI approach reveals how NP surface functionality affects host-guest interactions in cells, information difficult to achieve using other techniques. ? 2014 American Chemical Society.
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
ac501682y.pdf
Size
1.49 MB
Format
Adobe PDF
Checksum
(MD5):879c444213ff552154e814b1bb2a2302