Isolation and Characterization of New Sporamin Gene Members from Sweet Potato (Ipomoea batatas Lam.)
Journal
TAIWANIA
Journal Volume
42
Journal Issue
1
Pages
34-42
Date Issued
1997
Author(s)
Abstract
Two full-length and two partial cDNAs encoding sporamin A have been isolated from sweet potato tuberous roots. Sequence comparisons show that they are very similar with 94-98% homology at nucleotide level, and 80-88% at protein level. All four cDNAs possess multiple alternate polyadenylation signals in the 3' untranslated region (3'-UTR). Genomic Southern blot analysis indicates the presence of a sporamin multigene family in sweet potato. High levels of sporamin mRNAs were detected in developing tuberous roots, but they disappeared at the sprout-germinating stage. Differential expression of these genes was obvious as their mRNAs were present specifically in developing roots, rarely in stems and not in leaves.
SDGs
Type
journal article