Development of a Handheld Optical Sensing Meter with Disposable Test Strip for Fatigue Assessment
Date Issued
2011
Date
2011
Author(s)
Chou, Chih-Chi
Abstract
With the development and progress of modern society, fatigue and the related issues have gradually attracted public attention due to the pursuit of better quality of life. Therefore, the measurement of salivary α-amylase (sAA) has become an important issue in the field of psychoneuroendocrinology (PNE). According to previous studies, the secretion of sAA was significantly correlated with the physical and psychological stress induced by exercise, overworking or higher mental load. For this reason, sAA is regarded as an excellent biomarker comparing to the conventional measurement of electrophysiology and invasive biochemical examination. In this study, a colorimetric sensing device (λD = 525 nm) for determining the activity of sAA was developed for evaluation of the fatigue status.
For assembling a miniaturized optical system, electronic components such as light emitting diode, photodiode, constant current driver and several integrated circuits were used. Under the environment of LabVIEW, a computer program with graphical user interface was developed for data acquisition and signal conditioning. Biochemical reaction was conducted in a disposable test strip made of the polycarbonate (PC) substrate of a commercialized compact disc. The strips were fabricated with laser cutter and the sample volume was 180 µL. The detection time was 12 minutes for each sample. Based on the reduction in the absorbance caused by reducing sugar, the linear range for sAA detection was ranged from 0.1 to 1 U mL-1 and the calibration curve showed a regression coefficient of 0.949 and the sensitivity of 0.028 Hz / U mL-1, respectively. And the regression coefficient of system comparison with laboratory spectrophotometer was 0.995. The clinical data obtained with the proposed device were highly correlated with the serum cortical levels, the standard biochemical parameter for fatigue. This study provides an easy, fast, cost-effective and non-invasive way for fatigue evaluation which can help people for self-examination in the field of healthy life and road safety in the future.
Subjects
Handheld optical sensing meter
Salivary α-amylase
Fatigue measurement
Psychoneuroendocrinology
Reduce colorimetric assay
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-R98631013-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):7cbd292e01660ed1f0a5ba0ed7d309ad