The Applications of Cell-Pellets Forming and Protons Releasing in Microbial Bioremediation
Date Issued
2007
Date
2007
Author(s)
LIANG, MAO-SHIH
DOI
zh-TW
Abstract
To biodegrade hydrocarbon chemicals by means of microorganisms is a very noteworthy method in environmental engineering. Both NTU-1 Smooth and Rhodococcus erythropolis NTU-1 Rough can not only degrade hydrocarbons but also remove alkanes efficiently by trapping them in cell-pellets. Through this investigation, we found two methods to enhance the formation of cell-pellets. One is to add 2mL NB (Nutrient Broth) in 100mL MSM (Minimal Salt Medium), and the other is aeration. The former could effectively shorten pellet-forming period by one day. In addition, the increase of MSM buffer capacity was also observed to help NTU-1 Rough form cell pellets when utilizing octadecane.
According to this study, the amount of protons released by NTU-1 Rough, under moderate pH, was in proportion to the amount of n-alkane degraded. The ratios depend on the carbon numbers of those alkanes. This discovery provides a novel and easy way to monitor alkane quantity. With low pH, both NTU-1 Rough and NTU-1 Smooth still could utilize alkanes through different physiological mechanisms. The fact that both strains demonstrated bioremediation potential under low pH environment promises their future applications.
Evaluating microbial surface properties by a constant-pressure microfiltration found to be feasible. In our work, under low temperature and carbon starvation, the filtration specific resistances of both NTU-1 Rough and NTU-1 Smooth decreased with time dramatically. Especially after 15 days, specific resistance of NTU-1 Rough was reduced to one percent of its original level suggesting that the surface properties had enormous effects on the performance of filtration.
Subjects
生物復育
生物分解
氫離子
端過濾
bioremediation
biodegradation
proton
dead-end filtration
Type
thesis