Measurement of wavelength-dependent second order susceptibility tensor elements in types I and II collagens
Journal
Optik
Journal Volume
169
Pages
392-396
Date Issued
2018
Author(s)
Abstract
In this work, we measured the second order susceptibility (SOS) tensor ratios of χzzz/χzxx and χxzx/χzxx between 725–875 nm in type I (rat tail tendon) and II (rat trachea) collagen. In this wavelength range, χzzz/χzxx and χxzx/χzxx were found to be frequency-independent. Specifically, χzzz/χzxx and χxzx/χzxx are around 1.4 and 0.7 for collagen I; 1.2 and 0.4 for collagen II, respectively. Therefore, the near-infrared wavelength range of 700–800 nm is sufficiently far from the collagen resonance at 247 nm in order for the SOS tensor ratios to be wavelength independent. Our results suggest that microscopy imaging based on the use of SOS as a contract mechanism can use the simultaneous illumination of different excitation wavelengths with different polarizations for faster implementation of second order susceptibility microscopy.
SDGs
Type
journal article