HepG2肝細胞的電泳實驗與模擬
Electrophoresis of HepG2 Cells: Experimental and Theoretical Modeling
Date Issued
2005
Date
2005
Author(s)
Yang, Li-chia
DOI
zh-TW
Abstract
Electrophoresis, the movement of a charged entity as a response to an applied electric field, is a useful tool to gather information about the charged conditions on cell surface. The electrophoretic behavior of HepG2 cells is investigated in this study both experimentally and theoretically. Experimentally, Zetasizer3000 is used to determine the mobility of HepG2 cells. Effects of pH, ionic strength, and different kinds of divalent cations are examined. We can discuss the properties and the kinds of functional groups on the surface of HepG2 cells by electrophoresis. The result shows that absolute mobility increases with increasing pH, and decreases with increasing ionic strength. Because of different binding constants with surface functional group, there is different mobility with different kinds of divalent cation. Under pH 4.3, the mobility of HepG2 cells reverses to positive sign, and we can therefore infer that there are at least one kind of acid functional group and one kind of base functional group on the surface of HepG2 cells. There are two parts in the theoretical modeling. The first one the ion size in the electrolyte is ignored, and in the second it is considered. A charge-regulated model is proposed to simulate the charged conditions on cell surface, and to estimate the key parameters such as the thickness of the membrane layer, the density, the dissociation constant of dissociable functional groups in the membrane layer, and the binding constant of divalent cation. FlexPDE software is used to solve the governing equations. These data are compared to the experimental data in order to estimate the suitable parameters. The results can be used to explain the electrophoresis behavior of HepG2 cells.
Subjects
細胞電泳
表面性質
electrophoresis of cells
surface property
Type
thesis