Analgesic Effects and the Mechanisms of Anti-inflammation of Ergostatrien-3 beta-ol from Antrodia camphorata Submerged Whole Broth in Mice
Resource
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58(12), 7445-7452
Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Journal Volume
58
Journal Issue
12
Pages
7445-7452
Date Issued
2010
Date
2010
Author(s)
Huang, Guan-Jhong
Huang, Shyh-Shyun
Lin, Shiang-Shiou
Shao, Yi-Yuan
Chen, Chin-Chu
Hou, Wen-Chi
Kuo, Yueh-Hsiung
Abstract
Ergostatrien-3β-ol (ST1), an active and major ingredient from Antrodia camphorata (AC) submerged whole broth was evaluated for the analgesic and anti-inflammatory effects. Treatment of male imprinting control region (ICR) mice with ST1 (1, 5, and 10 mg/kg) significantly inhibited the numbers of acetic-acid-induced writhing response in 10 min. Also, our result showed that ST1 (10 mg/kg) significantly inhibited the formalin-induced pain in the late phase (p < 0.001). In the anti-inflammatory test, ST1 (10 mg/kg) decreased the paw edema at 4 and 5 h after λ-carrageenin (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. We also demonstrated that ST1 significantly attenuated the malondialdehyde (MDA) level in the edema paw at 5 h after Carr injection. ST1 (1, 5, and 10 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at 5 h after Carr injection. Also, ST1 (5 and 10 mg/kg) diminished the serum tumor necrosis factor (TNF-α) at 5 h after Carr injection. Western blotting revealed that ST1 (10 mg/kg) decreased Carr-induced inducible nitric oxide synthase (iNOS), and cycloxyclase (COX-2) expressions at 5 h in the edema paw. An intraperitoneal (ip) injection treatment with ST1 also diminished neutrophil infiltration into sites of inflammation, as did indomethacin (Indo). The anti-inflammatory mechanisms of ST1 might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver through the suppression of TNF-α and NO. ? 2010 American Chemical Society.
Subjects
Analgesic; Anti-inflammation; Chinese herb; Ergostatrien-3β-ol; MDA; NO; TNF-α
SDGs
Other Subjects
analgesic agent; antiinflammatory agent; carrageenan; cyclooxygenase 2; inducible nitric oxide synthase; malonaldehyde; analgesic agent; antiinflammatory agent; animal; Antrodia; article; chemically induced disorder; chemistry; disease model; drug effect; edema; gene expression; genetics; human; immunology; Institute for Cancer Research mouse; male; metabolism; mouse; pain; randomization; Antrodia; chemically induced; chemistry; drug effects; edema; pain; Analgesics; Animals; Anti-Inflammatory Agents; Antrodia; Carrageenan; Cyclooxygenase 2; Disease Models, Animal; Edema; Gene Expression; Humans; Male; Malondialdehyde; Mice; Mice, Inbred ICR; Nitric Oxide Synthase Type II; Pain; Random Allocation; Mus; Taiwanofungus camphoratus; Analgesics; Animals; Anti-Inflammatory Agents; Antrodia; Carrageenan; Cyclooxygenase 2; Disease Models, Animal; Edema; Gene Expression; Humans; Male; Malondialdehyde; Mice; Mice, Inbred ICR; Nitric Oxide Synthase Type II; Pain; Random Allocation
Type
journal article
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