The organ tropism of ovarian cancer metastasis
Date Issued
2014
Date
2014
Author(s)
Huang, Liang-Cheng
Abstract
Ovarian cancer is the most lethal gynecologic malignancy, and the majority of deaths occur due to metastatic disease. Many studies have identified the specific pattern of metastasis, termed organ tropism, including ovarian cancer.
In this thesis, we used the CB17/lcr-PRkdc scid/Crl mice to show that different ovarian cancer cell lines had different organ tropism. The A1847 line and A2780 line derived from primary ovarian tumors displayed ovary tropism upon intravenous injection. The Skov3 cells derived from the ascites of ovarian adenocarcinoma form metastases in lung upon intravenous injection. We analyzed the properties of ovarian cancer cells including morphology, migration ability, invasion ability, and proliferation in order to explain the different organ tropism. And we observed A1847 and A2780 cells metastasized to ovary 48 hours after intravenous injection, whereas Skov3 cells metastasized to lung tissue under the similar condition. When A1847 and A2780 cells were implanted directly into lung, they metastasized to ovary. Skov3 cells which were implanted orthotopically into ovary metastasized to lung.
It was shown that in breast cancer, circulating tumor cells self-seeded their tumor origin, we observed similar phenomenon in Skov3 cells. Accumulated clinical studies revealed 25% ovarian cancer cases involved bilateral ovarian carcinomas. We found that Skov3 cells were able to metastasize to contralateral ovary, and this was promoted by wound healing.
This thesis demonstrates the distinctive organ tropism of ovarian cancer cell lines, and the limiting step of metastasis resides more in successful colonization than initial targeting of the organs. Wounding healing could promote cancer cell colonization.
Subjects
卵巢癌
轉移
器官特異性
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-103-R01b43028-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):96394c1aa356cb3e5e691c0a902e3267