Immune bioactivity in shellfish toward serum-free cultured human cell lines
Journal
Bioscience, Biotechnology and Biochemistry
Journal Volume
61
Journal Issue
1
Pages
24-28
Date Issued
1997
Author(s)
Abstract
The biologically functional effect of eight kinds of hot-water extracts of shellfish on cultured human cell lines was examined in a serum-free medium model. Meretrix lusoria and Sinonovacula constricta extracts enhanced IgM secretion of both hybridoma HB4C5 and SI102 cells when cultured with the respective extracts. The purified principle exhibited remarked activity in the adsorbed fraction in hydroxyapatite and Concanavalin A columns. The extracts of Corbicula fluminea, Crassostreas gigas, Meretrix lusoria, Anadara granosa, and Sinonovacula constricta enhanced in nitroblue tetrazolium (NBT)-reducing ability of macrophage U-M cells. Meretrix lusoria, Anadara granosa, and Sinonovacula constricta were specifically cytotoxic to both cultures of MCF-7 breast cancer cells and HuH-6KK hepatoblastoma. These findings imply that the extracts of shellfish that were examined exhibited a differential effect on immune cells and tumor cells. © 1997, Taylor & Francis Group, LLC. All rights reserved.
Subjects
Antitumor; Bioactivity; Functional food; Immunity; Shellfish
Other Subjects
immunoglobulin M; nitroblue tetrazolium; tissue extract; water; animal; article; cell culture; cell division; chemistry; culture medium; drug effect; drug screening; fractionation; human; hybridoma; immunology; macrophage; metabolism; methodology; pathology; physiology; shellfish; Animals; Cell Division; Chemical Fractionation; Culture Media, Serum-Free; Drug Screening Assays, Antitumor; Humans; Hybridomas; Immunoglobulin M; Macrophages; Nitroblue Tetrazolium; Shellfish; Tissue Extracts; Tumor Cells, Cultured; Water
Type
journal article