Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array
Journal
Scientific Reports
Journal Volume
7
Journal Issue
1
Date Issued
2017
Abstract
Biomaterial-based tissue culture platforms have emerged as useful tools to mimic in vivo physiological microenvironments in experimental cell biology and clinical studies. We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PDMS)-based hanging drop array (PDMS-HDA) methodology. Multicellular spheroids can be achieved within 24 h and further boosted by incorporating collagen fibrils in PDMS-HDA. In addition, the spheroids generated from different human tumor cells exhibited distinct sensitivities toward drug chemotherapeutic agents and radiation as compared with two-dimensional (2D) cultures that often lack in vivo-like biological insights. We also demonstrated that multicellular spheroids may enable key hallmarks of tissue-based bioassays, including drug screening, tumor dissemination, cell co-culture, and tumor invasion. Taken together, these results offer new opportunities not only to achieve the active control of 3D multicellular spheroids on demand, but also to establish a rapid and cost-effective platform to study anti-cancer therapeutics and tumor microenvironments. ? 2017 The Author(s).
SDGs
Other Subjects
biocompatible coated material; dimeticone; bioassay; cell culture technique; coculture; human; multicellular spheroid; preclinical study; procedures; tumor cell line; Biological Assay; Cell Culture Techniques; Cell Line, Tumor; Coated Materials, Biocompatible; Coculture Techniques; Dimethylpolysiloxanes; Drug Evaluation, Preclinical; Humans; Spheroids, Cellular
Type
journal article