Detection of adenosine 5'-triphosphate by fluorescence variation of oligonucleotide-templated silver nanoclusters
Journal
Biosensors and Bioelectronics
Journal Volume
58
Pages
266-271
Date Issued
2014
Author(s)
Abstract
Oligonucleotide-templated Ag nanoclusters (DNA-Ag NCs) prepared from AgNO3 using an oligonucleotide (5'-TAACCCCTAACCCCT-3') as a template and NaBH4 as a reducing agent have been used for sensing of adenosine 5'-triphosphate (ATP). The fluorescence intensity and emission wavelength of DNA-Ag NCs are dependent on the pH value and ATP concentration. At pH 3.0 and 11.0, ATP shows greater effects on fluorescence of the DNA-Ag NCs. Upon increasing ATP concentration from 10 to 50μM, their emission wavelength at pH 3.0 shifts from 525 to 585nm. At pH 11.0, their fluorescence intensity (510nm) increases upon increasing ATP concentration. The circular dichroism (CD), electrospray ionization-mass spectrometry (ESI-MS), absorption, and fluorescence results indicate that ATP and pH affect the interactions between DNAs and Ag atoms, resulting in changes in their fluorescence. The DNA-Ag NCs allow detection of ATP over a concentration range of 0.1-10μM, with a limit of detection 33nM. Practicality of the DNA-Ag NCs probe has been validated with the determination of ATP concentrations in the lysate of MDA-MB-231 breast carcinoma cells. ? 2014 Elsevier B.V.
Subjects
Adenosine 5'-triphosphate; Ag nanoclusters; DNA structure; Fluorescence
SDGs
Other Subjects
DNA; Electrospray ionization; Fluorescence; Mass spectrometry; Nanoclusters; Oligonucleotides; pH effects; Ag nanoclusters; Breast carcinoma cells; Concentration ranges; DNA structure; Electrospray ionizationmass spectrometries (ESI-MS); Fluorescence intensities; Silver nanoclusters; Triphosphate; Silver; adenosine triphosphate; DNA; oligonucleotide; silver nanocluster; silver nanoparticle; silver nitrate; unclassified drug; adenosine triphosphate; metal nanoparticle; nanocomposite; silver; absorption; analytical parameters; article; breast carcinoma; cancer cell culture; cell lysate; cell strain MDA MB 231; chemical interaction; circular dichroism; concentration response; electrospray mass spectrometry; emission wavelength; fluorescence analysis; fluorescence intensity; limit of detection; molecular probe; pH; validation process; chemistry; device failure analysis; devices; equipment design; genetic procedures; genetics; molecular imprinting; nanotechnology; procedures; spectrofluorometry; ultrastructure; Adenosine Triphosphate; Biosensing Techniques; DNA; Equipment Design; Equipment Failure Analysis; Metal Nanoparticles; Molecular Imprinting; Nanocomposites; Nanotechnology; Silver; Spectrometry, Fluorescence
Type
journal article