Effects of ethyl acetate fraction from adlay bran ethanolic extract on the growth of human breast cancer cell
Date Issued
2010
Date
2010
Author(s)
Hsu, Chih-Ying
Abstract
In Taiwan, breast cancer is one of the most common malignancies in women. Epidemiological studies pointed out that female exposed to estrogen abnormally increased the incidence of breast cancer. However, phytoestrogen, a plant chemical with known estrogenic effects, can lower breast cancer risk. Phytosterols, a kind of phytoestrogens, including β-sitosterol and campesterol have anticancer and regulate the ability for estrogen expression. Adlay, an annual crop, has been reported with many health effect including anticancer. Previous researches indicated that many kinds of phytosterol exist in adlay bran. Therefore the aim of this study is to investigate if the extracts from adlay bran can sufficiently inhibit the growth of human breast cancer cells.
The results show that low polarity of adlay bran extract not only exhibited the most potential on inhibiting proliferation of breast cancer cell, MCF-7. But also shown non-toxicity to normal mammary epithelial cell, H184B5F5/M10. The flow cytometry was used to analyze cell cycle distribution. In the beginning, the 100 μg/ml extract could increase the probability of MCF-7 arrest at G0/G1 phase from 40% to 59%. After treating 16 hours, an obviously proportion of cells appeared in the sub-G1 phase. Apoptosis were detected by annexinV-PI stain. Treating MCF-7 with 100 μg/ml effective sample for 6 hours, the apoptosis percentage is 10 fold higher than untreatment group. Western blotting was used to detect several different protein expression.The results show that the express of p53 and p21 in breast cancer cell can be enhanced with treating extracts by increasing treat time. The amounts of phytosterols in different samples were quantified by LC-MS/MS. The analytical results shown that the amount of campesterol in extracts have positive relationship with their anticancer effect.
In conclusion, the low polarity of adlay bran extract induce apoptosis of MCF-7. Phytosterols in adlay bran can be active compounds to inhibit breast cancer cell proliferation.
Subjects
breast cancer
apoptosis
phytosterols
estrogen
SDGs
Type
thesis
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