Simple aminophenol based electrochemical probes for non enzymatic, dual amperometric detection of NADH and hydrogen peroxide
Journal
Talanta
Journal Volume
200
Pages
450 457
Date Issued
2019
Author(s)
Abstract
Non enzymatic detection of NADH and H2O2 is of practical significance for both environmental and biological prospective. However, there is no simple, straight forward electrochemical sensor available for sensing of them in real samples. Addressing this challenge, we report a simple stimuli responsive aminophenol, pre-anodized screen printed carbon electrode (SPCE*/AP) based electrochemical probes for dual detection of NADH and H2O2. Aminophenol prepared and adsorbed on the electrode from aminophenylboronic acid via boronic acid deprotection with H2O2. The SPCE*/AP fabricated with this process was characterized by cyclic voltammetry (CV), scanning electron microscope (SEM), Raman spectroscopy, UV-visible spectroscopy, and X-ray photoelectron spectroscopy (XPS). Amperometric detection results showed that SPCE*/AP electrodes exhibited linearity from 50 ?M to 500 ?M and from 200 ?M to 2 mM with a detection limit (S/N = 3) of 4.2 ?M and 28.9 ?M for NADH and H2O2, respectively. Excellent reproducibility and selectivity for NADH and H2O2 were observed for this electrochemical platform. In addition, the matrix effect was investigated further using the same technique to analyze NADH and H2O2 in human urine samples, human serum samples, cell culture medium (containing 10% fetal bovine serum, FBS), and environmental water samples (tap water and rain water). Also, the present sensor demonstrated promising outcomes with living cells (normal cells and cancer cells). ? 2019 Elsevier B.V.
Subjects
Aminophenol; Aminophenylboronic acid; Amperometric; H2O2; NADH; Preanodization; Screen printed carbon electrode
SDGs
Other Subjects
Body fluids; Cell culture; Cyclic voltammetry; Electrodes; Mammals; Probes; Scanning electron microscopy; Ultraviolet visible spectroscopy; X ray photoelectron spectroscopy; Aminophenol; Aminophenylboronic acid; Amperometric; H2O2; NADH; Preanodization; Screen-printed carbon electrodes; Electrochemical sensors; aminophenol derivative; carbon; fluorescent dye; hydrogen peroxide; nicotinamide adenine dinucleotide; 3T3 cell line; animal; chemistry; electrochemical analysis; electrode; human; mouse; tumor cell line; 3T3 Cells; Aminophenols; Animals; Carbon; Cell Line, Tumor; Electrochemical Techniques; Electrodes; Fluorescent Dyes; Humans; Hydrogen Peroxide; Mice; NAD
Type
journal article