Microsatellite instability confounds engraftment analysis of hematopoietic stem-cell transplantation
Journal
Applied Immunohistochemistry and Molecular Morphology
Journal Volume
22
Journal Issue
6
Pages
416-420
Date Issued
2014
Author(s)
Abstract
Polymorphic short tandem-repeat, or microsatellite, loci have been widely used to analyze chimerism status after allogeneic hematopoietic stem-cell transplantation. In molecular diagnostic laboratories, it is recommended to calculate mixed chimerism for at least 2 informative loci and to avoid microsatellite loci on chromosomes with copy number changes. In this report, we show that microsatellite instability observed in 2 patients with acute leukemia may confound chimerism analysis. Interpretation errors may occur even if 2 to 3 loci are analyzed because of length variation in multiple microsatellite loci. Although microsatellite loci with length variation should not be selected for chimerism analysis, the presence of microsatellite instability, like copy number alteration because of aberrant chromosomes, provides evidence of recurrent or residual cancer cells after hematopoietic stem-cell transplantation. Copyright ? 2013 by Lippincott Williams & Wilkins.
SDGs
Other Subjects
DNA; microsatellite DNA; acute leukemia; adult; allogeneic hematopoietic stem cell transplantation; analytical equipment; capillary electrophoresis; case report; chimera; chromosome aberration; comparative study; copy number variation; DNA isolation; engraftment; female; flow cytometry; gene locus; human; human cell; leukemia relapse; male; microsatellite instability; middle aged; priority journal; review; short tandem repeat; single nucleotide polymorphism; acute disease; allograft; animal; chimera; genetics; hematopoietic stem cell transplantation; human chromosome; leukemia; metabolism; pathology; Acute Disease; Allografts; Animals; Chimerism; Chromosomes, Human; Genetic Loci; Hematopoietic Stem Cell Transplantation; Humans; Leukemia; Microsatellite Instability; Microsatellite Repeats
Publisher
Lippincott Williams and Wilkins
Type
review