Hourglass-shaped aperture for cellular electrophysiological study
Journal
Applied Physics Letters
Journal Volume
91
Journal Issue
12
Date Issued
2007
Author(s)
Abstract
This work presents a simple process of reflow of melted glass by C O2 laser to form an hourglass-shaped aperture for cellular electrophysiological study. The fabricated aperture proves to be smooth, circular, debris-free, and freshly activated. Two-phase flow simulation illuminates details of the reflow process. The resulting seal resistance quality is well suited to measure ion-channel activities; a seal resistance of 1.9 G for PC-12 cell and a typical resistance of 200 M for Chinese hamster ovary cells were achieved. This approach can be integrated with microfluidics in a single cell patch or array configuration. © 2007 American Institute of Physics.
Type
journal article
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