Surface Modification of Ferrite Magnetic Nanoparticles and Application to Biomolecular Recognition
Date Issued
2004
Date
2004
Author(s)
Chou, Tzu-Chi
DOI
zh-TW
Abstract
In this thesis, we describe the preparation of magnetic nanoparticles linked with biomolecules, such as antibody, for specific recognition and easy separation of the target receptors, such as virus, and purified them.
As shown in the figure, three parts are connected. The first part is ferrite magnetic nanoparticles (NPs) which were covered with poly (acrylic acid) to prevent oxidation and to increase solubility in water. These acid groups are connected with the ethylene glycol type linkers with good water solubility. At the end of the linker, there is a dithiolpyridine moiety which can form a disulfide bond with the thiol groups on biomolecules. In the mean time of this sulfide exchange, 2-thiolpyridone is released and detected by UV absorption. This method is used to quantify the amount of attacked biomolecules. The biomolecules in my study include H6 avian influenza virus (AI virus) and its antibody. In the first approach, the virus is directly attacked on NPs. Alternatively, the virus-specific antibody is first attacked to the NPs, and then used to detect AI virus. Hemagglutination test is used to detect if there’s virus or not in the test sample.
Subjects
磁性
奈米
生物辨識
nano
magnetic
biomolecule recognition
Type
thesis
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