Use of fluorescein labelled antibody and fluorescence activated cell sorter for rapid identification of mycobacterium species
Journal
Biochemical and Biophysical Research Communications
Journal Volume
250
Journal Issue
2
Pages
403-408
Date Issued
1998
Author(s)
Yi W.-C.
Hsiao S.
Liu J.-H.
Soo P.-C.
Horng Y.-T.
Tsai W.-C.
Hsieh R.-F.
KWEN-TAY LUH
Ho S.-W.
Abstract
A fluorescein labelled antibody (Ab)/Fluorescence Activated Cell Sorter (FACS)-based assay was developed for detection of a wide range of mycobacterial species directly from bacterial culture and sputum specimens. The whole process could be completed within 3 hours and had a high specificity and sensitivity for cultured bacteria. The method was also shown to be applicable for direct identification from clinical specimens. This study showed that pretesting of clinical specimens for mycobacteria to the genus level with an antibody to mycobacterium species offers the routine clinical laboratory a single convenient test for the detection of tuberculous and nontuberculous mycobacteria. Depending on the availability of species-specific antibody, the identification of Mycobacterium to the species level can be achieved.
Other Subjects
antibody; fluorescein; article; bacterium culture; bacterium identification; controlled study; diagnostic accuracy; fluorescence activated cell sorter; human; Mycobacterium; Mycobacterium tuberculosis; nonhuman; priority journal; species difference; sputum; tuberculosis; Actinobacteria (class); Bacteria (microorganisms); Corynebacterineae; Mycobacterium; Mycobacterium tuberculosis; uncultured actinomycete
Type
journal article