Alginate-encapsulation of nodal segments for propagation, short-term conservation and germplasm exchange and distribution of Eclipta alba (L.)
Resource
ACTA PHYSIOLOGIAE PLANTARUM, 32(3), 607-610
Journal
Acta Physiologiae Plantarum
Pages
607-610
Date Issued
2010
Date
2010
Author(s)
Singh, Shashi Kant
Rai, Manoj K.
Asthana, Pooja
Sahoo, L.
Abstract
Nodal segments obtained from in vitro proliferated shoots of Eclipta alba (L.) Hassk, were encapsulated in calcium alginate beads for large-scale clonal propagation, short-term conservation and germplasm exchange and distribution. The best gel complexation was achieved using 3% sodium alginate and 100 mM CaCl2 2H2O. Maximum percent response (100%) for conversion of encapsulated nodal segments into plantlets was obtained on 0.7% agar-solidified full-strength MS medium containing 0.88 μMBAP. Encapsulated nodal segments could be stored at low temperature (4 C) up to 60 days with a survival frequency of 51.2%. The well-developed plantlets regenerated from encapsulated nodal segments were hardened-off successfully with 90% survival frequency. © Franciszek Góski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010.
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Type
journal article
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