Studies on Physiological and Biochemical Functions of Banana ABC (ATP-Binding Cassette) Transporter Genes
Date Issued
2010
Date
2010
Author(s)
Huang, Yu-Fen
Abstract
In order to understand the function of banana ABC transporter MhPDR1 and MhPDR2, ABC transporter (MhPDR1 or MhPDR2) overexpressed tobacco plants (Nt-MhPDR1 or Nt-MhPDR2) have been tested by several treatments. The results of heavy metals treatments indicated that Nt-MhPDR1 and Nt-MhPDR2 have higher tolerance to copper, cadmium, and zinc ions than untransformed plants. In heavy metal content analysis, the results showed that Nt-MhPDR1 and Nt-MhPDR2 accumulated lower concentrations of copper, cadmium, and zinc. In chemical component analysis, the results showed that Nt-MhPDR1 and Nt-MhPDR2 have higher chlorophyll and lower lipid peroxidation than untransformed plants. Therefore, banana ABC transporter MhPDR1 and MhPDR2 are suggested to play role as efflux pump of the copper, cadmium, and zinc ions. Furthermore, transgenic tobacco Nt-MhPDR1 and Nt-MhPDR2 accumulated higher nitrate, phosphate, potassium, and iron contents than untransformed plants. Therefore, MhPDR1 and MhPDR2 may also possibly play the role as a transporter for these elements. To investigate the cellular localization of ABC transporter MhPDR1 and MhPDR2, coding regions of MhPDR1 and MhPDR2 were fused with the green fluorescent protein (GFP) gene and transiently introduced into Arabidopsis protoplasts by PEG method. The result indicated that MhPDR1 and MhPDR2 are located at tonoplast and plasma membrane, respectively.
Subjects
ABC transporter
heavy metal
protein localization
Type
thesis
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