Expression of galactinol synthase genes of soybean under different abiotic stresses
Date Issued
2005
Date
2005
Author(s)
Liu, Chuarn-Yu
DOI
zh-TW
Abstract
Gene expression is induced when plant subjected to stresses, like drought and low temperature, and biochemical and physiological responses allow plant to adapt to the environmental stimuli. Raffinose family oligosaccharides (RFOs), for example, stachyose and raffinose, are of the biochemical product induced under stresses. In addition, RFOs also accumulate during seed maturation and participate in the drought tolerance of vegetative part and seeds.
Galactinol synthase (GolS) catalyzes the formation of galactinol from the substrates UDP-galactose and myo-inositol. RFOs accumulation was reported previously under low temperature in legume plants. The purpose of this study is to investigate the gene number of expressed GolS and their expression under different environmental stimuli. Sugar analysis of soybean leaf under drought indicates the accumulation of galactinol and raffinose, but not stachyose. These sugars, however, do not accumulate in the control plants. Accumulation of RFOs, especially stachyose, is also found in developing seeds. We have isolated two GolS genes (GmGolS 2 and GmGolS 2) from soybean leaves. GmGolS 2 is induced in root, stem and leaf under drought, low temperature and high salt, whereas GmGolS 1 is induced only in root under high salt.
Subjects
大豆
RFOs
GolS
逆境誘導
stress-inducibility
tissue-specific
Type
other
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