Real-time 3D particle manipulation visualized using volume holographic gratings
Journal
Optics Letters
Journal Volume
39
Journal Issue
10
Pages
3078-3081
Date Issued
2014
Author(s)
Abstract
Holographic optical tweezers (HOTs) extend optical trapping into three dimensions. Volume imaging then becomes a concern as trapped objects are easily moved out of focus of the imaging objective lens. Here we demonstrate a three-dimensional real-time interactive optical trapping, manipulating, and imaging system based on HOTs incorporated with volume holographic microscope. Intensity information about the trapped objects at multiple depths can be captured in a single measurement. This method is compatible with most imaging modes such as bright-field and fluorescence.
Type
journal article